Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavagePI Kulcsár, A Tálas, K Huszár, Z Ligeti, E Tóth, N Weinhardt, E Fodor, …Genome biology 18 (1), 1-17 2017
Mb-and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variantsE Tóth, BC Czene, PI Kulcsár, SL Krausz, A Tálas, A Nyeste, É Varga, …Nucleic acids research 46 (19), 10272-10285 2018
Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleasesE Tóth, É Varga, PI Kulcsár, V Kocsis-Jutka, SL Krausz, A Nyeste, …Nucleic acids research 48 (7), 3722-3733 2020
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′ G-extended sgRNAsPI Kulcsár, A Tálas, E Tóth, A Nyeste, Z Ligeti, Z Welker, E WelkerNature communications 11 (1), 1-14 2020
A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAsA Tálas, K Huszár, PI Kulcsár, JK Varga, É Varga, E Tóth, Z Welker, …Nucleic Acids Research 2021
BEAR reveals that increased fidelity variants can successfully reduce the mismatch-tolerance of adenine but not cytosine base editorsA Tálas, D Simon, P Kulcsár, É Varga, E Welker 2020